Yong Kun Park, M.D. Ph.D.
Médico patologista, bioquímico
professor da Faculdad de Ingeniería
de alimentos de UNICAMP.
Investigador visitante en diversos centros de investigación del mundo como; Rochester University, North Carolina University, Centro de control de enfermedades de las fuerzas armadas americanas, etc. Su principal interes es el uso de propoleos en biotecnologia e farmacologia.
Items 1 - 14 of 14
1: J Agric Food Chem. 2005 Dec 28;53(26):10306-9.
Suppressive effects of ethanolic extracts from propolis and its main botanical origin on dioxin toxicity.
Park YK, Fukuda I, Ashida H, Nishiumi S, Yoshida K, Daugsch A, Sato HH, Pastore GM.
Department of Food Science,
Suppressive effects of ethanolic extracts prepared from propolis group 12 and its main botanical origin (leaf bud of Baccharis dracunculifolia) on transformation of the aryl hydrocarbon receptor (AhR), the initial action of dioxin toxicity, were investigated. It was found that suppressive effects of propolis on AhR transformation were relatively higher than those of resins of its botanical origin in cell-free system and in Hepa-1c1c7 cells. When the composition of chemical ingredients was measured, propolis contained slightly higher amounts of flavonoid aglycones as compared with its botanical origin with the same characteristics. Moreover, antiradical activity, one of the typical biological activities of flavonoids, in propolis was also slightly higher than that in its botanical origin. These results indicate that not only propolis but also its botanical origin contains high amounts of flavonoid aglycones and that both of them are useful dietary sources for flavonoids with a potency to prevent dioxin toxicity.
PMID: 16366731 [PubMed - indexed for MEDLINE]
2: J Agric Food Chem. 2005 Feb 23;53(4):1166-72.
Some chemical composition and biological activity of northern Argentine propolis.
Isla MI, Paredes-Guzman JF, Nieva-Moreno MI, Koo H, Park YK.
Department of Food Science,
Twenty-five samples of propolis were collected from seven different regions in northern
PMID: 15713035 [PubMed - indexed for MEDLINE]
3: Biosci Biotechnol Biochem. 2004 Apr;68(4):935-8.
Suppression of dioxin mediated aryl hydrocarbon receptor transformation by ethanolic extracts of propolis.
Park YK, Fukuda I, Ashida H, Nishiumi S, Guzman JP, Sato HH, Pastore GM.
Present study demonstrated that the ethanolic extracts of propolis containing higher concentrations of flavonoids suppressed 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)-induced aryl hydrocarbon receptor transformation in a dose-dependent manner. The IC(50) values of propolis group 3 and group 12 were 1.2 and 3.6 microg/ml, respectively, indicating that propolis showed stronger antagonistic effects as compared with vegetable extracts.
4: J Agric Food Chem. 2004 Mar 10;52(5):1100-3.
Chemical constituents in Baccharis dracunculifolia as the main botanical origin of southeastern Brazilian propolis.
Park YK, Paredes-Guzman JF, Aguiar CL, Alencar SM, Fujiwara FY.
Department of Food Science,
Previously, it was reported that one group of propolis (Group 12) was identified in southeastern
PMID: 14995105 [PubMed - indexed for MEDLINE]
5: Oral Microbiol Immunol. 2002 Dec;17(6):337-43.
Effects of apigenin and tt-farnesol on glucosyltransferase activity, biofilm viability and caries development in rats.
Koo H, Pearson SK, Scott-Anne K, Abranches J, Cury JA, Rosalen PL, Park YK, Marquis RE, Bowen WH.
Center for Oral Biology and Eastman Department of Dentistry, University of
Propolis, a resinous hive product secreted by Apis mellifera bees, has been shown to reduce the incidence of dental caries in rats. Several compounds, mainly polyphenolics, have been identified in propolis. Apigenin and tt-farnesol demonstrated biological activity against mutans streptococci. We determined here their effects, alone or in combination, on glucosyltransferase activity, biofilm viability, and development of caries in rats. Sprague-Dawley rats were infected with Streptococcus sobrinus 6715 and treated topically twice daily as follows: (1) tt-farnesol, (2) apigenin, (3) vehicle control, (4) fluoride, (5) apigenin +tt-farnesol, and (6) chlorhexidine. Apigenin (
6: Caries Res. 2002 Nov-Dec;36(6):445-8.
Effect of a mouthrinse containing selected propolis on 3-day dental plaque accumulation and polysaccharide formation.
Koo H, Cury JA, Rosalen PL, Ambrosano GM, Ikegaki M, Park YK.
Department of Dentistry, University of
The aim of this study was to evaluate the effect of a mouthrinse containing propolis SNB-RS on 3-day dental plaque accumulation. Six volunteers took part in a double-blind crossover study performed in two phases of 3 days. During each phase the volunteers refrained from all oral hygiene and rinsed with 20% sucrose solution 5 times a day to enhance dental plaque formation and with mouthrinse (placebo or experimental) twice a day. On the 4th day, the plaque index (PI) of the volunteers was scored and the supragingival dental plaque was analyzed for insoluble polysaccharide (IP). The PI (SD) for the experimental group was 0.78 (0.17), significantly less than for the placebo group, 1.41 (0.14). The experimental mouthrinse reduced the IP concentration in dental plaque by 61.7% compared to placebo (p < 0.05). An experimental mouthrinse containing propolis SNB-RS was thus efficient in reducing supragingival plaque formation and IP formation under conditions of high plaque accumulation. Copyright 2002 S. Karger AG, Basel
PMID: 12459618 [PubMed - indexed for MEDLINE]
7: Antimicrob Agents Chemother. 2002 May;46(5):1302-9.
Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity.
Koo H, Rosalen PL, Cury JA, Park YK, Bowen WH.
Center for Oral Biology and Eastman Department of Dentistry, University of Rochester Medical Center, Rochester, New York 14642, USA. Hyun_Koo@urmc.rochester.edu
Propolis, a resinous bee product, has been shown to inhibit the growth of oral microorganisms and the activity of bacterium-derived glucosyltransferases (GTFs). Several compounds, mainly polyphenolics, have been identified in this natural product. The present study evaluated the effects of distinct chemical groups found in propolis on the activity of GTF enzymes in solution and on the surface of saliva-coated hydroxyapatite (sHA) beads. Thirty compounds, including flavonoids, cinnamic acid derivatives, and terpenoids, were tested for the ability to inhibit GTFs B, C, and D from Streptococcus mutans and GTF from S. sanguinis (GTF Ss). Flavones and flavonols were potent inhibitors of GTF activity in solution; lesser effects were noted on insolubilized enzymes. Apigenin, a 4',5,7-trihydroxyflavone, was the most effective inhibitor of GTFs, both in solution (90.5 to 95% inhibition at a concentration of 135 microg/ml) and on the surface of sHA beads (30 to 60% at 135 microg/ml). Antibacterial activity was determined by using MICs, minimum bactericidal concentrations (MBCs), and time-kill studies. Flavanones and some dihydroflavonols, as well as the sesquiterpene tt-farnesol, inhibited the growth of S. mutans and S. sobrinus; tt-farnesol was the most effective antibacterial compound (MICs of 14 to 28 microg/ml and MBCs of 56 to 112 microg/ml). tt-Farnesol (56 to 112 microg/ml) produced a 3-log-fold reduction in the bacterial population after 4 h of incubation. Cinnamic acid derivatives had negligible biological activities. Several of the compounds identified in propolis inhibit GTF activities and bacterial growth. Apigenin is a novel and potent inhibitor of GTF activity, and tt-farnesol was found to be an effective antibacterial agent.
PMID: 11959560 [PubMed - indexed for MEDLINE]
8: J Agric Food Chem. 2002 Apr 24;50(9):2502-6.
Botanical origin and chemical composition of Brazilian propolis.
Park YK, Alencar SM, Aguiar CL.
Department of Food Science,
Brazilian propolis has been classified into 12 groups based on physicochemical characteristics: five in the southern
PMID: 11958612 [PubMed - indexed for MEDLINE]
9: J Nat Prod. 2001 Oct;64(10):1278-81.
48.(1) Anti-HIV activity of moronic acid derivatives and the new melliferone-related triterpenoid isolated from Brazilian propolis.
Ito J, Chang FR, Wang HK, Park YK, Ikegaki M, Kilgore N, Lee KH.
Natural Products Laboratory,
A new triterpenoid named melliferone (1), three known triterpenoids, moronic acid (2), anwuweizonic acid (3), and betulonic acid (4), and four known aromatic compounds (5-8) were isolated from Brazilian propolis and tested for anti-HIV activity in H9 lymphocytes. Moronic acid (2) showed significant anti-HIV activity (EC(50) <0.1 microg/mL, TI >186) and was modified to develop more potent anti-AIDS agents.
PMID: 11678650 [PubMed - indexed for MEDLINE]
10: Caries Res. 2000 Sep-Oct;34(5):418-26.
Effects of Apis mellifera propolis on the activities of streptococcal glucosyltransferases in solution and adsorbed onto saliva-coated hydroxyapatite.
Koo H, Vacca Smith AM, Bowen WH, Rosalen PL, Cury JA, Park YK.
Faculty of Dentistry of
Propolis, a resinous hive product collected by Apis mellifera bees, has been used for thousands of years in folk medicine. Ethanolic extracts of propolis (EEP) have been shown to inhibit the activity of a mixture of crude glucosyltransferase (Gtf) enzymes in solution. These enzymes synthesize glucans from sucrose, which are important for the formation of pathogenic dental plaque. In the present study, the effects of propolis from two different regions of
11: Curr Microbiol. 2000 Sep;41(3):192-6.
Effect of a new variety of Apis mellifera propolis on mutans Streptococci.
Koo H, Rosalen PL, Cury JA, Ambrosano GM, Murata RM, Yatsuda R, Ikegaki M, Alencar SM, Park YK.
Department of Physiological Sciences, Faculty of Dentistry of Piracicaba, State University of Campinas, Caixa Postal 52, Piracicaba, 13414-900, SP, Brazil. Hyun-Koo@urmc.rochester.edu
The effects of a new variety of propolis, from Northeastern Brazil (BA), on growth of mutans streptococci, cell adherence, and water-insoluble glucan (WIG) synthesis were evaluated. Propolis from Southeastern (MG) and Southern (RS)
PMID: 10915206 [PubMed - indexed for MEDLINE]
12: Arch Oral Biol. 2000 Feb;45(2):141-8.
In vitro antimicrobial activity of propolis and Arnica
Koo H, Gomes BP, Rosalen PL, Ambrosano GM, Park YK, Cury JA.
Arnica and propolis have been used for thousands of years in folk medicine for several purposes. They possess several biological activities such as anti-inflammatory, antifungal, antiviral and tissue regenerative, among others. Although the antibacterial activity of propolis has already been demonstrated, very few studies have been done on bacteria of clinical relevance in dentistry. Also, the antimicrobial activity of Arnica has not been extensively investigated. Therefore the aim here was to evaluate in vitro the antimicrobial activity, inhibition of adherence of mutans streptococci and inhibition of formation of water-insoluble glucan by Arnica and propolis extracts. Arnica
13: Caries Res. 1999 Sep-Oct;33(5):393-400.
Effect of Apis mellifera propolis from two Brazilian regions on caries development in desalivated rats.
Koo H, Rosalen PL, Cury JA, Park YK, Ikegaki M, Sattler A.
Department of Physiological Sciences, Faculty of Dentistry of
The purpose of the present study was to evaluate the effect of Apis mellifera propolis collected from two regions of
14: Curr Microbiol. 1998 Jan;36(1):24-8.
Antimicrobial activity of propolis on oral microorganisms.
Park YK, Koo MH, Abreu JA, Ikegaki M, Cury JA, Rosalen PL.
Formation of dental caries is caused by the colonization and accumulation of oral microorganisms and extracellular polysaccharides that are synthesized from sucrose by glucosyltransferase of Streptococcus mutans. The production of glucosyltransferase from oral microorganisms was attempted, and it was found that Streptococcus mutans produced highest activity of the enzyme. Ethanolic extracts of propolis (EEP) were examined whether EEP inhibit the enzyme activity and growth of the bacteria or not. All EEP from various regions in