PROPOLEOS ANTIVIRAL
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Items 1 - 40 of 40 |
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1: Int J Antimicrob Agents. 2005
Nov;26(5):343-56. |
Antimicrobial activity of flavonoids.
Cushnie TP, Lamb AJ.
Flavonoids are ubiquitous in photosynthesising cells and are commonly found in
fruit, vegetables, nuts, seeds, stems, flowers, tea, wine, propolis and honey.
For centuries, preparations containing these compounds as the principal
physiologically active constituents have been used to treat human diseases.
Increasingly, this class of natural products is becoming the subject of
anti-infective research, and many groups have isolated and identified the
structures of flavonoids possessing antifungal, antiviral and antibacterial
activity. Moreover, several groups have demonstrated synergy between active
flavonoids as well as between flavonoids and existing chemotherapeutics.
Reports of activity in the field of antibacterial flavonoid research are widely
conflicting, probably owing to inter- and intra-assay variation in
susceptibility testing. However, several high-quality investigations have
examined the relationship between flavonoid structure and antibacterial
activity and these are in close agreement. In addition, numerous research
groups have sought to elucidate the antibacterial mechanisms of action of
selected flavonoids. The activity of quercetin, for example, has been at least
partially attributed to inhibition of DNA gyrase. It has also been proposed
that sophoraflavone G and (-)-epigallocatechin gallate inhibit cytoplasmic
membrane function, and that licochalcones A and C inhibit energy metabolism.
Other flavonoids whose mechanisms of action have been investigated include
robinetin, myricetin, apigenin, rutin, galangin,
2,4,2'-trihydroxy-5'-methylchalcone and lonchocarpol A. These compounds
represent novel leads, and future studies may allow the development of a
pharmacologically acceptable antimicrobial agent or class of agents.
Publication Types:
·
Review
PMID: 16323269 [PubMed - indexed for MEDLINE]
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2:
Leuk Res.
2005 Nov;29(11):1343-6. |
Evaluation of Manisa propolis effect on leukemia cell line by telomerase
activity.
Gunduz C, Biray C, Kosova B, Yilmaz B, Eroglu Z, Sahin F, Omay SB, Cogulu O.
Propolis is a resinous substance which is used by bees to repair and maintain
their hives. It has more than 180 compounds including flavonoids, phenolic
acids and its esters which have anti-inflammatory, antibacterial, antiviral,
immunomodulatory, antioxidant and antiproliferative effects. Propolis is shown
to inhibit cell division and protein synthesis. However the exact mechanism
underlying antitumor effect is not clearly described. On the other hand
progressive telomere shortening to a critical level results with senescence of
normal cells by inducing apoptosis and telomerase prevents erosion of
telomeres. In this study we aimed to evaluate hTERT ratios in propolis-treated
T-cell acute lymphoblastic leukemia (CCFR-CEM) cell line. Cell counts and cell
viability of propolis-treated and propolis-free T-cell acute lymphoblastic
leukemia (CCFR-CEM) cell line were assessed by trypan blue dye exclusion test
and MTT assay. The LightCycler instrument was used (online real-time PCR) for
the quantification of hTERT in CCFR-CEM cell line. The hTERT ratio
significantly decreased 60 and 93% after 24 and 72 h respectively compared to
the initial value of the cells incubated with propolis. It had almost no
cytotoxic effect and caused 30, 30, 22 and 12% decrease in cell counts after
24, 48, 72 and 96 h respectively which is statistically significant. In
conclusion propolis may show antitumor and apoptotic effect via inhibiting
telomerase expression besides the mechanisms which have been described
previously.
PMID: 16055186 [PubMed - indexed for MEDLINE]
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3: J Ethnopharmacol. 2005 Nov
14;102(2):158-63. Epub 2005 Jul 19. |
Anti-HIV-1 activity of propolis in CD4(+) lymphocyte and microglial cell
cultures.
Gekker G, Hu S, Spivak M, Lokensgard JR, Peterson PK.
Neuroimmunology Laboratory, Minneapolis Medical Research Foundation,
Minneapolis, MN 55415, USA; Centre for Infectious Diseases and Microbiology
Translational Research, University of Minnesota Medical School, Minneapolis, MN
55415, USA.
An urgent need for additional agents to treat human immunodeficiency virus type
1 (HIV-1) infection led us to assess the anti-HIV-1 activity of the natural
product propolis in CD4(+) lymphocytes and microglial cell cultures. Propolis
inhibited viral expression in a concentration-dependent manner (maximal
suppression of 85 and 98% was observed at 66.6mug/ml propolis in CD4(+) and
microglial cell cultures, respectively). Similar anti-HIV-1 activity was
observed with propolis samples from several geographic regions. The mechanism
of propolis antiviral property in CD4(+) lymphocytes appeared to involve, in
part, inhibition of viral entry. While propolis had an additive antiviral
effect on the reverse transcriptase inhibitor zidovudine, it had no noticeable
effect on the protease inhibitor indinavir. The results of this in vitro study
support the need for clinical trials of propolis or one or more of its
components in the treatment of HIV-1 infection.
PMID: 16046088 [PubMed - in process]
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4:
Neurosci
Lett. 2005 Jul 22-29;383(1-2):39-43. |
The flavanoide caffeic acid phenethyl ester blocks
6-hydroxydopamine-induced neurotoxicity.
Noelker C, Bacher M, Gocke P, Wei X, Klockgether T, Du Y, Dodel R.
Department of Neurology, Friedrich-Wilhelms-University,
Parkinson's disease (PD) is a neurodegenerative disorder characterized by
progressive loss of dopaminergic (DA) neurons of the substantia nigra pars
compacta. 6-Hydroxydopamine (6-OHDA) is specific to dopaminergic neurons in
intrastriatal rodent models. It induces neuronal death either via uncoupling
mitochondrial oxidative phosphorylation resulting in energy deprivation or
alternatively, is associated with its ability to produce hydrogen peroxide,
hydroxyl and superoxide radicals. Caffeic acid phenethyl ester (
PMID: 15894425 [PubMed - indexed for MEDLINE]
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5:
Clin
Biochem. 2005 Feb;38(2):191-6. |
Effects of caffeic acid phenethyl ester on lipid peroxidation and
antioxidant enzymes in diabetic rat heart.
Okutan H, Ozcelik N, Yilmaz HR, Uz E.
Department of Cardiovascular Surgery,
OBJECTIVES: The risk for cardiovascular disease is significantly high in
diabetes mellitus. Experimental evidence suggests that oxidative stress plays a
dominant role in the pathogenesis of diabetes mellitus. Caffeic acid phenethyl
ester (
PMID: 15642285 [PubMed - indexed for MEDLINE]
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6:
Medicina
(Kaunas). 2004;40(8):771-4. |
[Investigation of chemical composition of propolis extract]
[Article in Lithuanian]
Majiene D, Trumbeckaite S, Grunoviene D, Ivanauskas L, Gendrolis A.
Institute for Biomedical Research,
Propolis is a natural product, produced by bees and containing exudates from
plants, mixed with bee wax. Propolis and its ethanolic extract are usually used
for treatment and prevention of different diseases. Propolis has antibacterial,
antiviral, antifungal, anti-inflammatory, anesthetic and immunomodulating
properties. Till now there is no data about chemical composition of Lithuanian
propolis. Thus, the aim of our work was to investigate the chemical composition
of Lithuanian propolis and its ethanolic extract by using gas chromatography /
mass spectrometry. We found, that the main structural types of compounds were
terpenoids, aromatic and aliphatic acid esters. The most of terpenoids were
mono- and sesquiterpens: azulene, alpha-bisabolol, citral, valerenol, etc.
Thus, our data show, that the composition of propolis is various and depends on
the origin of plants, from where propolis was collected.
PMID: 15299997 [PubMed - indexed for MEDLINE]
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7: Free Radic Biol Med. 2004 Aug
1;37(3):386-94. |
Protective effects of caffeic acid phenethyl ester against experimental
allergic encephalomyelitis-induced oxidative stress in rats.
Ilhan A, Akyol O, Gurel A, Armutcu F, Iraz M, Oztas E.
Department
of Neurology,
Because oxidative damage has been known to be involved in inflammatory and
autoimmune-mediated tissue destruction, modulation of oxygen free radical
production represents a new approach to the treatment of inflammatory and autoimmune
diseases. Central nervous system tissue is particularly vulnerable to oxidative
damage, suggesting that oxidation plays an important role in the pathogenesis
of multiple sclerosis (MS) and its animal model, experimental autoimmune
encephalomyelitis (EAE). Caffeic acid phenethyl ester (
PMID: 15223072 [PubMed - indexed for MEDLINE]
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8:
Int
Immunopharmacol. 2004 Mar;4(3):429-36. |
Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice.
Park JH, Lee JK, Kim HS, Chung ST, Eom JH, Kim KA, Chung SJ, Paik SY, Oh HY.
Immunotoxicology Division, Department of Toxicology, National Institute of
Toxicological Research, KFDA, Nokbun-Dong, Eunpyung-Gu, Seoul 122-704, South
Korea. jaehyun@kfda.go.kr
Caffeic acid phenethyl ester (CAPE), an the active component of propolis, is
known to have anticarcinogenic, antiviral and various biological activities;
however, the effect of
PMID: 15037220 [PubMed - indexed for MEDLINE]
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9: Asia Pac J Clin Nutr. 2003;12
Suppl:S66. |
Spasmolysis of combined bee glue and Shaoyao-Gancao-tang on isolated
rabbit jejunum.
Wang NZ, Li D.
Dept of Food Science,
Background - Propolis (bee glue) is a valuable byproduct of apiculture, which
is found in honeybee hives. It has been suggested that propolis-ethanol-extract
has antitumour, antimicrobial, antifungal, antiviral, antihepatotoxic,
antioxidative, and anti-inflammatory activities. Shaoyao-Gancao-tang, a
prescription of Chinese Medicine, consists of water extract of liquorice (Radix
Glycyrrhizac) and peony (Paeonialaciflora) roots. Objective - To investigate
the effect of combined propolis-ethanol-extract and Shaoyao-Gancao-tang (PSG)
on contraction of isolated rabbit jejunum in vitro. Design - PGS was prepared
from the ethanol extract of propolis and water extract of 1:1 liquorice and
peony roots. One end of the jejunum tube (3-4cm) was fastened to the
ventilating pipe of a thermostatic bath, the other end to the tensioning
exchanger, which was connected to a Recorder. Outcomes - With the same
concentration (0.02%), PSG showed a 31% reduction on the maximum relaxation
time compared with propolis-ethanol-extracts or Shaoyao-Gancao-tang (P<0.01,
n= 10). This result indicates that there was a synergistic effect between
propolis-ethanol-extracts and Shaoyao-Gancao-tang on the myenteric relaxation
of jejunum in vitro. Conclusions - The action of PSG on jejunum smooth muscles
is most likely through M-receptors, since acetylcholine induced jejunum
contractions were significantly decreased by PSG, and there was a remarkable
synergistic effect between atropine and PSG (P<0.001, n= 8). In conclusions,
the PGS showed an antispasmodic activity, and inhibits peristalsis of jejunum
smooth muscles via inhibiting M-receptors.
PMID: 15023697 [PubMed - in process]
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10:
Clin
Chim Acta. 2003 Dec;338(1-2):11-6. |
Protective role of Egyptian propolis against tumor in mice.
El-khawaga OA, Salem TA, Elshal MF.
Chemistry Department, Faculty of Science,
BACKGROUND: Propolis has numerous biologic activities including antibiotic,
antifungal, antiviral and anti-inflammatory properties. The present work is
aimed to study the effect of crude Egyptian propolis on tumor in mice induced
by Ehrlich ascitis carcinoma (EAC) cell line. RESULTS: The administration of
propolis (160 mg/kg body weight), by gastric intubation 2 h before the
intraperitoneal injection of EAC, effectively inhibited tumor growth and the proliferation
of EAC. The tumor volume was markedly reduced from 7+/-0.9 ml in EAC-infected
mice to 1.6+/-0.95 ml in propolis-treated mice. Also, the lipid peroxide level
which was 13.3+/-1.24 nmol malodialdehyde (MDA)/mg protein in EAC infected mice
was significantly decreased to 3.3+/-2.1 nmol MDA/mg protein. Reduced
glutathione (GSH) and glutathione S-transferase (GST) concentrations were
markedly increased in propolis-treated mice. This effect was associated with
inhibition of cell cycle progression and induction of apoptosis. Administration
of propolis 2 h before injection of EAC arrested cells in G0/G1 phase and
resulted in a decrease in the viability, DNA, total RNA and protein level of
tumor cells. CONCLUSIONS: Crude Egyptian propolis has a strong inhibitory
activity against tumors. The anti-tumor mechanism may be mediated by preventing
oxidative damage and induction of apoptosis.
PMID: 14637260 [PubMed - indexed for MEDLINE]
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11:
Biochem
Pharmacol. 2003 Dec 15;66(12):2281-9. |
Involvement of tumor suppressor protein p53 and p38 MAPK in caffeic acid
phenethyl ester-induced apoptosis of C6 glioma cells.
Lee YJ, Kuo HC, Chu CY, Wang CJ, Lin WC, Tseng TH.
Department of Chemistry,
Caffeic acid phenethyl ester (
PMID: 14637186 [PubMed - indexed for MEDLINE]
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12:
Fitoterapia.
2002 Nov;73 Suppl 1:S44-52. |
In vitro permeation through porcine buccal mucosa of caffeic acid
phenetyl ester (
Ceschel GC, Maffei P, Sforzini A, Lombardi Borgia S,
Yasin A, Ronchi C.
Dipartimento di Scienze Farmaceutiche, Universita di Bologna, Via San Donato
19/2, 4100, Bologna, Italy. Paolam@biofarm.unibo.it
Recent studies have shown that propolis has on the oral cavity appreciable
antibacterial, antifungal and antiviral actions, as well as anti-inflammatory,
anaesthetic and cytostatic properties. In light of these studies, an assessment
of the diffusion and permeation of caffeic acid phenetyl ester (
PMID: 12495709 [PubMed - indexed for MEDLINE]
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13:
Fitoterapia.
2002 Nov;73 Suppl 1:S21-9. |
Antioxidant activity of propolis: role of caffeic acid phenethyl ester
and galangin.
Russo A, Longo R, Vanella A.
Department of Biochemistry, Medical Chemistry and Molecular Biology, University
of Catania, V.le A. Doria 6, 95125, Catania, Italy. alrusso@mbow.unict.it
Propolis, a natural product produced by the honeybee, has been used for
thousands of years in folk medicine for several purposes. The extract contains
amino acids, phenolic acids, phenolic acid esters, flavonoids, cinnamic acid,
terpenes and caffeic acid. It possesses several biological activities such as
antiinflammatory, immunostimulatory, antiviral and antibacterial. The exact
mode of physiological or biochemical mechanisms responsible for the medical
effects, however, is yet to be determined. In this work, we have investigated
the antioxidant activity of a propolis extract deprived of caffeic acid
phenethyl ester (
PMID: 12495706 [PubMed - indexed for MEDLINE]
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14: Isr Med Assoc J. 2002 Nov;4(11
Suppl):923-7. |
Anti-herpes simplex virus effect of an aqueous extract of propolis.
Huleihel M, Isanu V.
Institute for Applied Biosciences,
BACKGROUND: Propolis, a natural product from beehives, comprises a complex of
chemicals, the most important group being flavinoids, which play a role in
antiviral protection. OBJECTIVES: To test the inhibitory effect of propolis
extract against herpes simplex viruses in vitro and in vivo. METHODS: In vitro:
propolis was added to Vero cells at various times and concentrations before, at
or after infection with HSV-
PMID: 12455181 [PubMed - indexed for MEDLINE]
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15: Isr Med Assoc J. 2002 Nov;4(11
Suppl):919-22. |
Comment in:
·
Isr Med Assoc J. 2002 Nov;4(11
Suppl):944-6.
The effect of herbal remedies on the production of human inflammatory
and anti-inflammatory cytokines.
Barak V, Birkenfeld S, Halperin T, Kalickman I.
Immunology Laboratory for Tumor Diagnosis, Department of Oncology,
BACKGROUND: Some herbal remedies are sold as food additives and are believed to
have immune-enhancing properties. OBJECTIVES: To study the effect of five
herbal remedies--Sambucol Black Elderberry Extract, Sambucol Active Defense
Formula and Sambucol for Kids (with known antiviral properties), Protec and
Chizukit N (containing propolis and Echinacea, claimed to be immune
enhancers)--on the production of cytokines, one of the main components of the
immune system. METHODS: The production of four inflammatory cytokines
(interleukin-1 beta, tumor necrosis factor alpha, and IL-6 and IL-8) and one
anti-inflammatory cytokine (IL-10) was tested using blood-derived monocytes
from 12 healthy donors. RESULTS: The Sambucol preparations increased the
production of five cytokines (1.3-6.2 fold) compared to the control. Protec
induced only a moderate production of IL-8 (1.6 fold) and IL-10 (2.3 fold)
while Chizukit N caused only a moderate increase in IL-10 production (1.4
fold). Both Protec and Chizukit N caused moderate decreases in IL-1 beta, TNF
alpha and IL-6 production. Lipopolysaccharide, a known activator of monocytes,
induced the highest levels of cytokine production (3.6-10.7 fold). CONCLUSIONS:
The three Sambucol formulations activate the healthy immune system by
increasing inflammatory and anti-inflammatory cytokines production, while the
effect of Protec and Chizukit N is much less. Sambucol could therefore have
immunostimulatory properties when administered to patients suffering from
influenza (as shown before), or immunodepressed cancer or AIDS patients who are
receiving chemotherapy or other treatments.
PMID: 12455180 [PubMed - indexed for MEDLINE]
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16:
Z
Naturforsch [C]. 2002 Mar-Apr;57(3-4):386-94. |
Egyptian propolis: 2. Chemical composition, antiviral and antimicrobial
activities of
Abd El Hady FK, Hegazi AG.
Department of Chemistry of Natural Products,
Three propolis samples from East Nile Delta,
PMID: 12064745 [PubMed - indexed for MEDLINE]
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17:
Anticancer
Res. 2001 May-Jun;21(3B):1665-71. |
PM-
Luo J, Soh JW, Xing WQ, Mao Y, Matsuno T, Weinstein IB.
Herbert
Irving Comprehensive Cancer Center, Columbia Presbyterian Medical Center,
College of Physicians and Surgeons, Columbia University, New York, NY 10032,
USA.
Propolis has numerous biologic activities including antibiotic, antifungal,
antiviral and anti-inflammatory properties. Several components isolated from
propolis have been shown to have anticancer activity. This study demonstrates
that the compound PM-3
(3-[2-dimethyl-8-(3-methyl-2-butenyl)benzopyran]-6-propenoic acid) isolated
from Brazilian propolis markedly inhibits the growth of MCF-7 human breast
cancer cells. This effect was associated with inhibition of cell cycle
progression and induction of apoptosis. Treatment of MCF-7 cells with PM-3
arrested cells in the G1 phase and resulted in a decrease in the protein levels
of cyclin D1 and cyclin E. PM-3 also inhibited the expression of cyclin D1 at
the transcriptional level when examined in cyclin D1 promoter luciferase
assays. Induction of apoptosis by PM-3 occurred within 48 hours after treatment
of MCF-7 cells. The MCF-7 treated cells also displayed a decrease in the level
of the estrogen receptor (ER) protein and inhibition of estrogen response
element (ERE) promoter activity. Therefore, PM-3 merits further investigation
with respect to breast cancer chemoprevention or therapy.
PMID: 11497245 [PubMed - indexed for MEDLINE]
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18:
Phytomedicine.
2000 Mar;7(1):1-6. |
A comparative multi-centre study of the efficacy of propolis, acyclovir
and placebo in the treatment of genital herpes (HSV).
Vynograd N, Vynograd I, Sosnowski Z.
Ninety men and women with recurrent genital HSV type 2 participated in a
randomized, single-blind, masked investigator, controlled multi-centre study
comparing the efficacy of ointment of Canadian propolis containing natural
flavonoids with ointments of acyclovir and placebo (vehicle) on healing ability
and capacity to remedy symptoms. Thirty individuals were randomized to each
group. Treatment was intended to start in the blister phase. All participants
had HSV type 2 isolated, confirmed by serum immunoglobulin levels. The
participants were examined on the 3rd, 7th and 10th days of treatment by
gynaecologists, dermatovenerologists or urologists at seven different medical
centres. Apart from clinical symptoms the number and size of the herpetic
lesions were noted. At each examination the lesions were classified into four
stages: vesicular, ulcerated, crusted and healed. The study ointments were
applied to affected areas four times daily. In women with vaginal or cervical
lesions a tampon with the appropriate ointment was inserted four times daily
for 10 days. Endpoint variables were healing time and time until loss of
symptoms. RESULTS: On Day 10, 24 out of 30 individuals in the propolis group
had healed. In the acyclovir group 14 out of 30 and in the placebo group 12 out
of 30 had healed. (p = 0.0015). The healing process appeared to be faster in
the propolis group. In the propolis group 15 individuals had crusted lesions on
Day 3 compared to 8 individuals in the acyclovir group and none in the placebo
group (p = 0.0006). On Day 7, 10 participants in the propolis group,
Publication Types:
PMID: 10782483 [PubMed - indexed for MEDLINE]
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19:
Arch
Oral Biol. 2000 Feb;45(2):141-8. |
In vitro antimicrobial activity of propolis and Arnica
Koo H, Gomes BP, Rosalen PL, Ambrosano GM, Park YK, Cury JA.
Arnica and propolis have been used for thousands of years in folk medicine for
several purposes. They possess several biological activities such as
anti-inflammatory, antifungal, antiviral and tissue regenerative, among others.
Although the antibacterial activity of propolis has already been demonstrated,
very few studies have been done on bacteria of clinical relevance in dentistry.
Also, the antimicrobial activity of Arnica has not been extensively
investigated. Therefore the aim here was to evaluate in vitro the antimicrobial
activity, inhibition of adherence of mutans streptococci and inhibition of
formation of water-insoluble glucan by Arnica and propolis extracts. Arnica
PMID: 10716618 [PubMed - indexed for MEDLINE]
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20: Z Naturforsch [C]. 1999
Jul-Aug;54(7-8):549-53. |
Synergism between ethanolic extract of propolis (EEP) and
anti-tuberculosis drugs on growth of mycobacteria.
Scheller S, Dworniczak S, Waldemar-Klimmek K, Rajca M, Tomczyk A, Shani J.
Department of Microbiology and Immunology,
Ethanolic extract of propolis exerts a strong anti-bacterial activity, in
addition to antifungal, antiviral and antiprotozoal properties. In previous
studies from these laboratories we have demonstrated that the intensity of the
bactericidal activity of EEP is correlated with the virulence of the
mycobacteria tested, and that EEP has a synergistic effect with antibiotics on
growth of staphylococcus aureus. In the present study we investigated whether
the same synergism and correlation exists between EEP and some
anti-tuberculosis drugs on tuberculosis mycobacteria with different degrees of
virulence. Six standard strains and 11 wild strains of mycobacteria were
exposed for 30 days to EEP, with or without streptomycin, rifamycin, isoniazid
or ethambutol. Out of the 17 strains, 8 were resistant to at least two standard
antibiotics, and were considered "multi-resistant strains". The rest
were either susceptible or resistant to only one of the antimycobacterial
drugs. Antagonism was recorded only in one case, when Staphylococcus aureus
were treated with a mixture of EEP and ethambutol, suggesting that a chemical
bond could have been formed between this anti-tuberculosis antibiotic and one
of the active components of the ethanol extract of propolis.
PMID: 10488563 [PubMed - indexed for MEDLINE]
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21: Int J Mol Med. 1999 Jul;4(1):29-32. |
Apoptosis induced by propolis in human hepatocellular carcinoma cell
line.
Choi YH, Lee WY, Nam SY, Choi KC, Park YE.
Department of Pathology,
Propolis has been reported to exhibit a wide spectrum of activities including
antibiotic, antiviral, anti-inflammatory, immunostimulatory and tumor
carcinostatic properties. We showed propolis induced apoptosis in a human
hepatoma cell line (SNU449) by FITC-Annexin V/PI staining. We also compared the
apoptosis inducing effect between Korean and Commercial (Sigma # p-1010)
propolis. There was no difference on apoptosis between them.
PMID: 10373633 [PubMed - indexed for MEDLINE]
|
22:
J
Ethnopharmacol. 1999 Mar;64(3):235-40. |
Antibacterial, antifungal and antiviral activity of propolis of
different geographic origin.
Kujumgiev A, Tsvetkova I, Serkedjieva Y, Bankova V, Christov R, Popov S.
Propolis samples from different geographic origins were investigated for their
antibacterial (against Staphylococcus aureus and Escherichia coli), antifungal
(against Candida albicans) and antiviral (against Avian influenza virus)
activities. All samples were active against the fungal and Gram-positive
bacterial test strains, and most showed antiviral activity. The activities of
all samples were similar in spite of the differences in their chemical
composition. In samples from the temperate zone, flavonoids and esters of
phenolic acids are known to be responsible for the above mentioned activities
of bee glue; tropical samples did not contain such substances but showed
similar activities. Obviously, in different samples, different substance combinations
are essential for the biological activity of the bee glue. It seems that
propolis has general pharmacological value as a natural mixture and not as a
source of new powerful antimicrobial, antifungal and antiviral compounds.
PMID: 10363838 [PubMed - indexed for MEDLINE]
|
23: Z Naturforsch [C]. 1998
Nov-Dec;53(11-12):1040-4. |
Correlation between virulence of various strains of mycobacteria and
their susceptibility to ethanolic extract of propolis (EEP).
Scheller S, Kawalski H, Oklek K, Dworniczak S, Matsuno T, Waldemar-Klimmek K, Rajca M, Shani J.
Department of Microbiology and Immunology,
Ethanol extract of propolis (EEP) has antibacterial, antiviral, antiprotozoal
and antifungal properties, in addition to many biological effects. Our
laboratory has demonstrated a synergistic effect of EEP and antibiotics on the
growth of Staphylococcus aureus, and suggested that the bactericidal effect of
EEP was expressed mainly on virulent mycobacteria rather than on non-virulent
(attenuated) ones. The present study was designed to reconfirm the latter
finding, by subjecting 17 different mycobacteria strains to EEP, and evaluating
whether there is a correlation between the virulence of the mycobacteria
strains studied and their susceptibility to EEP. Our findings demonstrate that
while the four non-virulent strains studied are not susceptible to EEP, out of
the 13 virulent strains studied seven are susceptible and six are resistant to
it. These results suggest that while there is no full correlation between
virulence of the mycobacteria tested and their susceptibility to EEP, the few
strains that were resistant to EEP were non-virulent.
PMID: 9933968 [PubMed - indexed for MEDLINE]
|
24: Rom J Virol. 1993
Jan-Jun;44(1-2):49-54. |
[Mechanism of the anti-herpetic activity of aqueous extract of propolis.
II. Activity of lectins from the aqueous extract of propolis]
[Article in French]
Dumitrescu M, Crisan I, Esanu V.
Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
The report brings proofs of the presence of a lectin in the water propolis
extract. It was detected in human fibroblast extracts previously treated with
the propolis extract. Presence of the lectin was confirmed by polyacrylamide
gel electrophoresis in the presence of SDS.
PMID: 9702248 [PubMed - indexed for MEDLINE]
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25:
Food
Chem Toxicol. 1998 Apr;36(4):347-63. |
Review of the biological properties and toxicity of bee propolis
(propolis).
Burdock GA.
Burdock and Associates,
Propolis is a multifunctional material used by bees in the construction and
maintenance of their hives. Use of propolis by humans has a long history,
predated only by the discovery of honey. Use of products containing propolis
have resulted in extensive dermal contact and it is now increasingly being used
a dietary supplement. Unlike many 'natural' remedies, there is a substantive
database on the biological activity and toxicity of propolis indicating it may
have many antibiotic, antifungal, antiviral and antitumour properties, among
other attributes. Although reports of allergic reactions are not uncommon,
propolis is relatively non-toxic, with a no-effect level (NOEL) in a 90-mouse
study of 1400 mg/kg body weight/day.
Publication Types:
·
Review
PMID: 9651052 [PubMed - indexed for MEDLINE]
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26:
Drugs
Exp Clin Res. 1997;23(2):89-96. |
Suppression of HIV-1 replication by propolis and its immunoregulatory
effect.
Harish Z, Rubinstein A, Golodner M, Elmaliah M, Mizrachi Y.
In the current study we show that propolis, a non-toxic natural bee-hive
product, suppresses HIV-1 replication and modulates in vitro immune responses.
CEM cells were treated with propolis at nontoxic concentrations prior to or
following infection with HIV-1. Propolis abolished syncytium formation at 4.5
micrograms/ml and inhibited it at lower doses in a concentration-dependent
manner. Propolis decreased p24 antigen production by as much as 90-100% in a
concentration-dependent manner. Furthermore, modulation of peripheral blood
mononuclear cells (PBMCs) mitogenic responses upon the addition of propolis was
noted, reducing the elevated responses to Concanavalin A (Con A) and enhancing
suppressed mitogenic responses to pokeweed mitogen (PWM). In summary, propolis
may constitute a non-toxic natural product with both anti HIV-1 and
immunoregulatory effects.
PMID: 9309384 [PubMed - indexed for MEDLINE]
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27:
Voen
Med Zh. 1995 Dec;(12):36-9, 80. |
[The use of ocular drug films of propolis in the sequelae of ophthalmic
herpes]
[Article in Russian]
Maichuk IuF, Orlovskaia LE, Andreev VP.
There was studied the therapeutic efficiency of ocular medical propolis films
(OMF) in 35 patients with postherpetic trophic keratitis and in 20 with
postherpetic nebula. OMF were applied behind the lower eyelid at bedtime during
10-15 days. All the patients endured the propolis films well. OMF accelerated
the cornea epithelization. Epitheliopathy and micropoint edema of cornea
epithelium rapidly disappeared. Time of patients recovery reduced nearly twice
(P,001) in comparison with the control group--from 14.1 to 7.6 days. On the
average their visual acuity increased in two times--from 0.12 to 0.27 (P >
0.001).
PMID: 8779164 [PubMed - indexed for MEDLINE]
|
28:
Boll
Chim Farm. 1995 Oct;134(9):483-91. |
[Propolis: its use in technology and research]
[Article in Italian]
Gallo FR, Savi G.
Borsista presso l'ISS nel laboratorio di
Chimica del Farmaco Viale Regina Elena, Roma, Italy.
Analytical
procedure more advanced allowed to identify, to isolate, and assay the
compounds present in propoli which is is developed to be a valuable biological
product as food resource as medical resource. Analytical procedure more and
more sophisticated allowed (us) to assay the propoli composition without
operate the derivatization of phenolic constituents making use the capacity of
different detector used. These phenolic contents were analysed by capillary gas
chromatography using an electron-capture detector. This detector shown a good
electron capture response of these compounds, which belong to the so-called
"conjugated electrophores".
Publication Types:
·
Review
PMID: 8527100 [PubMed - indexed for MEDLINE]
|
29:
J Nat
Prod. 1994 May;57(5):644-7. |
Comparison of the anti-herpes simplex virus activities of propolis and
3-methyl-but-2-enyl caffeate.
Amoros M, Lurton E, Boustie J, Girre L, Sauvager F, Cormier M.
Laboratoire de Pharmacognosie et Mycologie.
The in
vitro activity against herpes simplex virus type 1 of 3-methyl-but-2-enyl
caffeate isolated from poplar buds or prepared by synthesis was investigated.
Under conditions of one or multiple multiplication cycles, this compound, which
is a minor constituent of propolis, was found to reduce the viral titer by 3
log10, and viral DNA synthesis by 32-fold.
PMID: 8064297 [PubMed - indexed for MEDLINE]
|
30: Rev Roum Virol. 1993
Jan-Jun;44(1-2):49-54. |
[The mechanism of the antiherpetic action of an aqueous propolis extract.
II. The action of the lectins of an aqueous propolis extract]
[Article in French]
Dumitrescu M, Crisan I, Esanu V.
Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
The report brings proofs of the presence of a lectin in the water propolis
extract. It was detected in human fibroblast extracts previously treated with
the propolis extract. Presence of the lectin was confirmed by polyacrylamide
gel electrophoresis in the presence of SDS.
PMID: 8043477 [PubMed - indexed for MEDLINE]
|
31:
J Nat
Prod. 1992 Dec;55(12):1732-40. |
Synergistic effect of flavones and flavonols against herpes simplex
virus type
Amoros M, Simoes CM, Girre L, Sauvager F, Cormier M.
Laboratoire de Pharmacognosie et Mycologie,
Faculte de Pharmacie, Universite de Rennes I, France.
The in
vitro activity against herpes simplex virus type 1 of the major flavonoids
identified in propolis was investigated. Flavonols were found to be more active
than flavones, the order of importance being galangin, kaempferol, and
quercetin. The efficacy against HSV-1 of binary flavone-flavonol combinations
has been also investigated. The synergy demonstrated by all combinations could
explain why propolis is more active than its individual compounds.
PMID: 1338212 [PubMed - indexed for MEDLINE]
|
32:
Chem
Biol Interact. 1992 Nov 16;84(3):277-90. |
Effect of caffeic acid esters on carcinogen-induced mutagenicity and
human colon adenocarcinoma cell growth.
Rao CV, Desai D, Kaul B, Amin S, Reddy BS.
Division of Nutritional Carcinogenesis, American Health Foundation,
Propolis, a honey bee hive product, is thought to exhibit a broad spectrum of
activities including antibiotic, antiviral, anti-inflammatory and tumor growth
inhibition; some of the observed biological activities may be due to caffeic
acid (cinnamic acid) esters that are present in propolis. In the present study
we synthesized three caffeic acid esters, namely methyl caffeate (MC),
phenylethyl caffeate (PEC) and phenylethyl dimethylcaffeate (PEDMC) and tested
them against the 3,2'-dimethyl-4-aminobiphenyl, (DMAB, a colon and mammary
carcinogen)-induced mutagenicity in Salmonella typhimurium strains TA 98 and TA
100. Also, the effect of these agents on the growth of human colon
adenocarcinoma, HT-29 cells and activities of ornithine decarboxylase (ODC) and
protein tyrosine kinase (PTK) was studied. Mutagenicity was induced in
Salmonella typhimurium strains TA 98 and TA 100 plus S9 activation using 5 and
10 micrograms DMAB and antimutagenic activities of 0-150 microM MC, 0-60 microM
PEC and 0-80 microM PEDMC were determined. The results indicate that MC, PEC
and PEDMC were not mutagenic in the Salmonella tester system. DMAB-induced
mutagenicity was significantly inhibited with 150 microM MC, 40-60 microM PEC
and 40-80 microM PEDMC in both tester systems. Treatment of HT-29 colon
adenocarcinoma cells with > 150 microM MC, 30 microM PEC and 20 microM PEDMC
significantly inhibited the cell growth and syntheses of RNA, DNA and protein.
ODC and PTK activities were also inhibited in HT-29 cells treated with
different concentrations of MC, PEC and PEDMC. These results demonstrate that
caffeic acid esters which are present in Propolis possess chemopreventive
properties when tested in short-term assay systems.
PMID: 1423745 [PubMed - indexed for MEDLINE]
|
33:
Rev
Roum Virol. 1992 Jul-Dec;43(3-4):165-73. |
[The mechanisms of the antiherpetic action of aqueous propolis extracts.
I. The antioxidant action on human fibroblast cultures]
[Article in French]
Dumitrescu M, Esanu V, Crisan I.
Institut de Virologie Stefan S. Nicolau, Bucarest, Roumanie.
A redox state modulation model was worked out in human fibroblast cultures
treated with oxidation stress inducing agents and a redox agent, virtually
protecting cell against the stress. Quantification of the global redox changes
in fibroblasts was done using the hemoglobin electronic spectrum, in the
presence and in the absence of H2O2.
PMID: 1339205 [PubMed - indexed for MEDLINE]
|
34:
J Nat
Prod. 1992 Mar;55(3):294-302. |
Anti-influenza virus effect of some propolis constituents and their
analogues (esters of substituted cinnamic acids).
Serkedjieva J, Manolova N, Bankova V.
The antiviral activity of six synthetic substances, esters of substituted
cinnamic acids, identical with or analogous to some of the constituents of the
Et2O fraction of propolis was studied in vitro. One of them, isopentyl
ferulate, inhibited significantly the infectious activity of influenza virus
A/Hong Kong (H3N2) in vitro and the production of hemagglutinins in ovo. By the
use of diverse experimental patterns, it was found that the maximal inhibition
of viral reproduction was observed when test substances were present in the
medium during the whole infectious process.
PMID: 1593279 [PubMed - indexed for MEDLINE]
|
35:
Experientia.
1988 Mar 15;44(3):230-2. |
Preferential cytotoxicity on tumor cells by caffeic acid phenethyl ester
isolated from propolis.
Grunberger D, Banerjee R, Eisinger K, Oltz EM, Efros L, Caldwell M, Estevez V, Nakanishi K.
The honeybee hive product, propolis, is a folk medicine employed for treating
various ailments. Many important pharmaceutical properties have been ascribed
to propolis, including anti-inflammatory, antiviral, immunostimulatory and
carcinostatic activities. Propolis extracts have provided an active component
identified as caffeic acid phenethyl ester (
PMID: 2450776 [PubMed - indexed for MEDLINE]
|
36:
Acta
Microbiol Bulg. 1988;23:52-7. |
[The chemical composition of propolis fractions with antiviral action]
[Article in Bulgarian]
Bankova V, Popov S, Marekov N, Manolova N, Maksimova V.
PMID: 3247872 [PubMed - indexed for MEDLINE]
|
37:
Acta
Microbiol Bulg. 1985;17:79-85. |
[Antiviral action of fractions isolated from propolis]
[Article in Bulgarian]
Maksimova-Todorova V, Manolova N, Gegova G, Serkedzhieva Iu, Uzunov S.
PMID: 3834770 [PubMed - indexed for MEDLINE]
|
38:
Virologie.
1984 Oct-Dec;35(4):281-93. |
Research in the field of antiviral chemotherapy performed in the
"Stefan S. Nicolau" Institute of Virology.
Esanu V.
A brief review is made of the research in the field of antiviral chemotherapy
performed in the "Stefan S. Nicolau"
Publication Types:
PMID: 6097022 [PubMed - indexed for MEDLINE]
|
39:
Virologie.
1981 Jan-Mar;32(1):57-77. |
Recent advances in the chemotherapy of herpes virus infections.
Esanu V.
The main categories of antiherpes agents presently used in chemotherapy area
reviewed according to the phase of virus replication affected : 1) virus
adsorption (adamantane, nonionic surfactants) ; 2) eclipse (interferon) ; 3)
virion maturation (nucleoside and nucleotide analogues and phosphonic acid
derivatives). Mention is also made of other compounds--different synthetic
organic derivatives, photodynamic dyes, metal ions, boric acid, hormones,
antibiotics, other natural products (extracts from marine algae, propolis,
garlic)--with promising antiviral properties. The difficulties and prospects of
viral chemotherapy research are briefly discussed.
Publication Types:
·
Review
PMID: 6191437 [PubMed - indexed for MEDLINE]
|
40:
Zahn
Mund Kieferheilkd Zentralbl. 1978;66(5):470-3. |
[Experience obtained in the treatment of recurrent aphthous stomatitis
(author's transl)]
[Article in German]
Baikowa RA, Terjochowa NW.
Three different methods employed within the framework of complex therapy were
used to treat 195 patients with recurrent aphthous stomatitis: 1. Alterative
therapy using prodigiosan, a bacterial polysaccaride. 2. Desensitizing
treatment using histaglobulin, an antihistaminic agent. 3. Symptomatic local
treatment. The methods of treatment are described in detail. General therapy
allowed the frequency of relapses and the duration of disease to be reduced and
shortened, respectively. The use of a complex form of therapy is recommended.
PMID: 31043 [PubMed - indexed for MEDLINE]